一株抗硅藻附着的海绵附生菌的鉴定及发酵条件优化          下载免费PDF全文

刘书宇  俞思羽  夏翊腾  靳翠丽  张立奎  周晓见 《应用海洋学学报》2016,35(4):499-506

为了从海绵共附生微生物中寻找天然高效抗污损活性物质,本文针对前期研究获取的4株具有显著抗硅藻附着的活性菌株进行活性验证和比较,选出其中最优菌株进行菌种鉴定及发酵条件优化.比较了活性菌株对小新月菱形藻(Nitzschia closterium)、咖啡双眉藻(Amphora coffeaeformis)、碎片菱形藻(Nitzschia frustulum)附着的抑制活性,确定菌株UST050418-715为最优菌株;通过16S r DNA的测序分析,结合平板的菌落形态和扫描电镜下菌体特征,鉴定活性菌株UST050418-715为短小芽孢杆菌(Bacillus pumilus).为优化该菌株的培养条件,选择了酵母粉、蛋白胨、Na Cl、p H值进行四因素三水平的正交实验,对菌株培养条件进行优化.发现选择的4个因素对应的菌株发酵产物对硅藻附着的抑制活性大小不同,影响显著性顺序是酵母粉蛋白胨Na Clp H值,综合菌株发酵产物活性、粗提物产量和菌株生长情况进行分析,确定菌株UST050418-715基于海洋2216E培养基的最佳发酵条件为:蛋白胨含量7.5 g/dm3,酵母粉含量3 g/dm3,Na Cl含量10.45 g/dm3,p H值9.5.  相似文献   

Living the good life? The rise of urban mixed-use enclaves in Metro Manila     

Jana Maria Kleibert  Lisa Kippers 《Urban geography》2016,37(3):373-395

Gated communities and other forms of privatized enclaves have become features of many cities. The rise of urban mixed-use enclaves, which integrate functions of work, entertainment and living, is a relatively new and understudied phenomenon that is expanding rapidly throughout the Global South. In this article, we advance the concept of enclave urbanism through a case study of Eastwood City in Metro Manila, the Philippines. We trace the enabling factors for mixed-use enclave creation and assess how the different functions are marketed and integrated in practice into one “live–work–play” development. Based on extensive qualitative research, we argue that the global capital accumulation strategy of real estate developers leads to a strong focus on production and entertainment functions, but neglects questions of liveability. We reflect on the implications of our findings for mega-urban regions and urban studies research.  相似文献   

Catching sea cucumber fever in coastal communities: Conceptualizing the impacts of shocks versus trends on social-ecological systems     

《Global Environmental Change》2017

Research on vulnerability and adaptation in social-ecological systems (SES) has largely centered on climate change and associated biophysical stressors. Key implications of this are twofold. First, there has been limited engagement with the impacts of social drivers of change on communities and linked SES. Second, the focus on climate effects often assumes slower drivers of change and fails to differentiate the implications of change occurring at different timescales. This has resulted in a body of SES scholarship that is under-theorized in terms of how communities experience and respond to fast versus slow change. Yet, social and economic processes at global scales increasingly emerge as ‘shocks’ for local systems, driving rapid and often surprising forms of change distinct from and yet interacting with the impacts of slow, ongoing ‘trends’. This research seeks to understand the nature and impacts of social shocks as opposed to or in concert with trends through the lens of a qualitative case study of a coastal community in Mexico, where demand from international seafood markets has spurred rapid development of a sea cucumber fishery. Specifically, we examined what different social-ecological changes are being experienced by the community, how the impacts of the sea cucumber fishery are distinct from and interacting with slower ongoing trends and how these processes are affecting system vulnerability, adaptations and adaptive capacity. We begin by proposing a novel framework for conceptualizing impacts on social systems, as comprised of structures, functions, and feedbacks. Our results illustrate how the rapid-onset of this fishery has driven dramatic changes in the community. New challenges such as the ‘gold-rush-style’ arrival of new actors, money, and livelihoods, the rapid over-exploitation of fish stocks, and increases in poaching and armed violence have emerged, exacerbating pressures from ongoing trends in immigration, overfishing and tourism development. We argue that there is a need to better understand and differentiate the social and ecological implications of shocks, which present novel challenges for the vulnerability and adaptive capacity of communities and the sustainability of marine ecosystems.  相似文献   

广东沿岸海域发生赤潮的硅藻种类   总被引：8，自引：0，他引：8  

李永振 《台湾海峡》2001,20(2):274-278

根据1990－1991年在广东沿岸海域采集的178份浮游植物样品的鉴定，产结合近几年在广东沿海河口湾海域几次浮游植物采样的分析资料，报告了广东沿岸海域硅藻赤潮生物47种，逐种列出了其生态习性和地理分布及在广东沿岸海域的分布情况。  相似文献   

南海北部15 kaBP以来表层海水温度变化:来自海洋硅藻的记录   总被引：3，自引：0，他引：3  

黄元辉  黄玥  蒋辉 《海洋地质与第四纪地质》2007,27(5):65-74

对西太平洋边缘海148个表层硅藻样品中菱形海线藻(Thalassionema nitzschioides)及其小形变种(Thalassionema nitzschioidesvar.parva)的分布与表层海水温度(SST)之间关系进行研究,将菱形海线藻小形变种与所有菱形海线藻百分含量的比值(简称硅藻Rparva值)视为温度指数,开展南海北部MD05-2904孔附近海域15kaBP以来表层海水温度变化研究。结果表明:(1)南海北部15 kaBP以来表层海水温度呈现明显的阶段性波动特点,即末次冰消期气候转暖过程中,12.9~11.5 kaBP前后温度略有下降,与新仙女木事件对应,全新世南海北部气候依次经历了升温期、高温期和降温期3个变化阶段;(2)全新世大暖期鼎盛期结束后,南海北部海水温度阶段下降,两次明显降温分别发生于4 kaBP前后和1.5 kaBP前后;(3)MD05-2904孔硅藻Rparva值时间序列曲线与董哥洞D4石笋δ18O曲线对比表明,南海北部表层海水温度变化与末次冰消期以来东亚季风强度变化关系较为密切。  相似文献   

Zooplanktonic Communities of the Divergence Zone in the Northwestern Alboran Sea     

Jaime  Rodríguez  Alberto  García Valeriano  Rodriguez 《Marine Ecology》1982,3(2):133-142

Abstract. Using principal component analysis, the zooplanktonic communities of the divergence zone in the northwestern sector of the Alboran Sea were studied. The study was based on 35 vertical trawls in the 200 metres superficial level. The two first components account for 65 % of the total variance. The first component is a contrast between a warm water coastal community (characterized by low specific diversity and the abundance of Cladocera and Copepoda such as Acartia clausi, Centropages chierchiae and Temora stylifera) and a community of cold subsuperficial water distinguished by high specific diversity and a low number of individuals, one of its most characteristic elements being the eggs and larval stages of the Conostomasidae Maurolicus muelleri. The second component appears to be associated with an ecotone between the communities previously cited, and with a specific grouping (Rhincalanus nasutus, Eucalanus monachiis, Pseudocalanus elongatus, Temora longicornis , etc.) resulting from the peripheral displacement of subsuperficial elements brought to the surface by the cyclonic circulation of water masses between the Atlantic current and the Spanish coast.  相似文献   

南海深海表层沉积硅藻的分布   总被引：10，自引：1，他引：10  

陆钧 《海洋地质与第四纪地质》2001,21(2):27-30

定量分析了南海36个深海现代表层沉积样品中的硅藻，发现主要的种有Thalassionema nitzschilides、Chaetoceros messanensis、Coscinodiscus modulifer、Thalassionthrix longissima、Nitzschia marina和Cyclotella spp.。根据硅藻优势种含量、丰度等特征以及Q型聚类分析，大致划分为5个组合带，它们主要受温度、上升流等环境因素的影响。  相似文献   

Gradient responses of epilithic diatom communities in the Baltic Sea proper   总被引：1，自引：0，他引：1  

Anna Ulanova  Pauli Snoeijs   《Estuarine, Coastal and Shelf Science》2006,68(3-4):661

Diatom communities and assemblages are widely used as indicators of ecological change in aquatic environments and for reconstructing palaeo-environments. Good calibration data sets, directly linking changes in diatom composition to environmental factors, are needed for building reliable gradient models with high indicative value. Such models have a broad applicability because most diatom species have cosmopolitan distributions. This paper presents community analyses of brackish-water diatoms living on submerged stones in four areas of the Baltic Sea proper along the Swedish coast. The communities on the stones were composed of epilithic, epiphytic, epipelic, epipsammic and occasionally some pelagic species. Altogether, 158 quantitative samples were taken at 41 sites between 23 April and 11 May, 1990, and the data set contained 300 diatom taxa belonging to 75 genera. Species data were analysed with principal components analysis (PCA) and environmental factors were fitted passively by multiple regression analysis on the ordination results. Differences in community composition could be explained by variation in salinity (which was correlated with N:P and Si:P ratios and the occurrence of macroalgae on the stones), nutrient concentrations and variation in exposure to wave action (which was correlated to the occurrence of soft bottoms around the stones, water temperature and the occurrence of sand grains and macroalgae on the stones). Separate analyses for small taxa (cell biovolume <1000 μm³), for large taxa (cell biovolume ≥1000 μm³) and for small and large diatom taxa together showed that diatoms of different size classes respond differently to environmental variation.  相似文献   

日照沿海开发对潮间带生境及底栖动物群落的影响     

高翔  徐敬明 《海洋科学集刊》2002,(44):61-65

日照市位于山东省东南的黄海之滨，海岸线长99.6km，沿海滩涂面积约51km2。自1983年起，笔者每年都带学生到日照沿海进行专业实习。本文是作者对近20年实习资料的总结。结果发现：由于沿海的开发利用，潮间带动物的群落结构发生巨变，生物种群数量正逐年减少。  相似文献   

杂色鲍苗大规模脱落死亡的初步研究     

王瑞旋  刘广锋  徐力文  王江勇  黎伟坚  陈毕生 《海洋科学》2007,31(4):53-57

研究了杂色鲍(Haliotis diversicolorReeve)鲍苗脱落死亡周期中水质、硅藻和细菌变化情况。结果显示,鲍苗脱落前各水质指标无明显变化,脱落前硅藻数量大幅度增加,以卵形藻(Cocconeisspp.)和舟形藻(Naviculaspp.)为主要种类。同时发现一株可疑致病菌,初步鉴定为溶藻弧菌(Vibrio alginolyticus)。实验表明水质等相关因子与鲍苗脱落无直接关系,而硅藻很可能是致病菌的“载体”,整个附着膜微环境与鲍苗存亡息息相关。  相似文献